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. 2021 Mar 25;12:1756. doi: 10.1038/s41467-021-22010-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Image showing high-density culture of 5700 cells/mm². Tight junction protein ZO-1 is shown in magenta, Lamin A in green, and DAPI in blue. Scalebar, 15 µm. b Enlarged images of the region shown by the orange ROI in (h). DAPI is shown in blue and Lamin A is shown in color-coded intensity. Scale bar, 10 µm. c Image showing low density culture of 70 cells/ mm². Tight junction protein ZO-1 is shown in magenta, Lamin A in green, and DAPI in blue. Scalebar, 15 µm. d Enlarged images of the region shown by orange ROI in (j). DAPI is shown in blue, and Lamin A is shown in color-coded intensity. Scale bar, 10 µm. e Binned scatter plot between D_cell and D_nuc generated by binning D_cell in bins of 0.07. Each point in the plot represents mean value of D_cell and D_nuc in the bin. The red points represent data from HD culture and cyan points represent data from LD culture. The data are presented as mean ± S.E.M. Solid red line represents the linear regression to the data. Pearson’s correlation coefficient R_p is 0.97. f Box-plot showing LamA in high density (HD) and low density (LD) cultures. The sample number, n represents the number of cells analyzed over 3 independent biological replicates. g Binned scatter plot between D_nuc and LamA intensity generated by binning D_nuc in bins of 0.06. Each point in the plot represents mean value of D_nuc and LamA in the bin. The red points represent data from HD culture and cyan points represent data from LD culture. The data are presented as mean ± S.E.M. Solid black line represents the LOESS regression to the data. Spearman’s rank correlation coefficient R_sp is 0.87 In the box-plot, horizontal line represents the median of the data, lower and upper bounds of the box represent the 25th and 75th percentile of data, and the whiskers represent the minimum and maximum of the data. The scattered point on the box represents the actual data points. p values are estimated by two-sided Student’s t-test and the p values are indicated in the respective plots.