FIGURE 3.

AtSAUR32 loss-of-function and gain-of-function effect on Arabidopsis plant under drought stress conditions. (A) Transcript levels of AtSAUR32 in wild type (WT) and AtSAUR32-overexpressed lines (OE32-5, OE32-6, and OE32-10). (B) Survival rates of WT and AtSAUR32-overexpressed lines (OE32-5 and OE32-6), 1 week after rewatering. (C) Transpiration water loss from WT, saur32, and OE32-5 Arabidopsis plant leaves at various time points after detachment. Leaves of similar developmental stage were detached and weighted at the mentioned time; leaves’ initial weight was used as a fresh weight to calculate water loss. (D) Stoma aperture of leaves of WT, saur32, and OE32-5 Arabidopsis plants under 10 μM ABA treatment. (E) Proline accumulation. (F) SOD content in WT, saur32, and OE32-5 plants. (G) Phenotypes showed DAB (3,3-diaminobenzidine) staining, indicating H₂O₂ accumulation after drought treatment. Mean values and standard deviation (+SD) were plotted as analyzed by Duncan’s multiple range (DMR) test (P < 0.05); lowercase letters (a–d) and asterisk (*) indicate significant difference.