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Box plots showing no difference in the number of GL SMI-32 stained axonal torpedoes between groups (A) even after adjusting for age and gender (C). A significant decrease in GL SMI-34-ir torpedo numbers was observed in DS compared to HC and AD cases (B; Kruskal–Wallis, p < 0.001). When adjusted for age and gender, GL SMI-34-ir axonal torpedo counts were significantly reduced in DS compared to AD, but not HC (D; ANCOVA, p < 0.001). * denotes significance between groups.
