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. 2021 Feb 22;4(5):2000224. doi: 10.1002/adtp.202000224

Figure 4.

Figure 4

ITZ and EB inhibited authentic SARS‐CoV‐2 virus entry in vitro. a,b) Inhibition of SARS‐CoV‐2 infection by a) ITZ and b) EB. Vero‐E6 cells were pretreated with gradient concentrations of ITZ or EB for 1 hour and infected by SARS‐CoV‐2 at MOI  =  0.05. Viral infection (red) and cell viability (bule) were determined by using RT‐qPCR and CCK‐8 assays, respectively. c‐f), There images per concentration of ITZ (c,d) or EB (e,f) were acquired, as detected by using plaque assay. CQ (10 µM) was made as a positive control. (Data are represent as mean ± SD of the percentage infection relative to Mock, n = 3), Immunofluorescence images of g) ITZ or h) EB dose‐dependently inhibiting SARS‐CoV‐2 N protein expression (blue: DAPI; green: SARS‐CoV‐2 N protein). CQ (10 µm) was made as a positive control. Scale bars: 200 µm.