Figure 4.

MAVRICS is compatible with wastewater surveillance and detection of other human pathogenic viruses. RNA was extracted by MAVRICS or by the QIAamp viral RNA mini kit (Qiagen kit) from A,B) wastewater concentrate samples spiked with SARS‐CoV‐2 RNA and intact murine noroviruses (MNVs). C,D) wastewater biomass immobilized on electronegative membranes with SARS‐CoV‐2 RNA spike‐in. A, C) the SARS‐CoV‐2 RNA copy numbers were calculated by the standard curve method. The recovery of viral RNA was compared to the input amount. B,D) MNV and PPMoV copy numbers in the original sample were compared between MAVRICS and Qiagen kit. E) RNA was extracted using MAVRICS or the TRIzol reagent protocol from a human respiratory pathogens control panel (influenza A and B viruses, and respiratory syncytial virus (RSV)). The Ct was obtained using a clinical diagnostic rRT‐PCR panel to quantitate the viruses. TRIzol+ and SiMNP+: positive control panel containing pathogens. TRIzol‐ and SiMNP‐: blank control panel without microorganism. Data are shown as mean ± SEM of 3 A,D), 6 B SiMNP), 2 B Qiagen kit), 6 C), or 5 E) technical replicates in one PCR assay per sample.