Table 1.
List of materials for SiMNP synthesis, RNA extraction and rRT‐PCR (The vendors and catalog numbers are those used in this study. Alternative sources can also be used)
| Reagent | Supplier | Catalog number |
|---|---|---|
| Iron (II) chloride tetrahydrate ≥98% (FeCl2 ·4 H2O) | VWR Chemicals | 13478‐10‐9 |
| Iron (III) chloride, anhydrous, extra pure (FeCl3) | Fisher Scientific | 10224390 |
| Sodium hydroxide, ≥99% (NaOH) | Sigma Aldrich | 306576‐500G |
| Hydrochloric acid (36.5 to 38.0%) | Fisher Scientific | A144‐500 |
| Ammonia solution (NH4OH, 25%) | Fisher Scientific | 10642251 |
| Ethanol absolute ≥99.8% | VWR Chemicals | 20821.330 |
| Tetraethyl orthosilicate (≥99%) (GC) | Sigma Aldrich | 78‐10‐4 |
| 2,2‐bis(hydroxymethyl)‐2,2′,2″‐nitrilotriethanol/bis‐tris (C8H19NO5) | Gold Biotechnology | B‐020‐500 |
| Guanidinium chloride/Gu‐HCl (CH5N3 · HCl) | Fisher Scientific | BP178‐1 |
| Tris base | Promega Corporation | H5135 |
| Tween 20 | Sigma Aldrich | P7949 |
| TRIzol reagent | Life Technologies | 15596018 |
| SuperScript IV reverse transcriptase | Invitrogen | 18090010 |
| RNase OUT | Invitrogen | 10777‐019 |
| TaqMan Fast Advanced Master Mix | Invitrogen | 4444556 |
| RNase H | New England BioLabs | M0297L |
| 2019‐nCoV Kit | Integrated Device Technology (IDT) | 10006605 |
| Influenza/RSV qPCR assay | Integrated Device Technology (IDT) | 1079729 |
| Direct‐Zol RNA Miniprep kit | Zymo Research | R2070 |
| QIAamp viral RNA mini kit | Qiagen | 52906 |
| ProtoScript II reverse transcriptase | New England BioLabs | M0368 |