TABLE 1.
Predicted siRNAs for the leader sequence, corresponding target sequences and seed duplex stability parameters or T m values of respective strands
|
Leader sequence ACCUUCCCAGGUAACAAACCAACCAACUUUCGAUCUCUUGUAGAUCUGUUCUCUAAACGAAC | |||||
|---|---|---|---|---|---|
| S. no. | Target position | Target sequence |
RNA oligo sequences 21 nt guide (5′→3′) 21 nt passenger (5′→3′) along with 2‐nt 3′ overhangs |
Seed‐duplex stability (T m value °C) | |
| Guide | Passenger | ||||
| 1 | 13‐35 | AACAAACCAACCAACUUUCGAUC |
UCGAAAGUUGGUUGGUUUGUU CAAACCAACCAACUUUCGAUC |
19.7 | 18.8 |
| 2 | 25‐47 | AACUUUCGAUCUCUUGUAGAUCU |
AUCUACAAGAGAUCGAAAGUU CUUUCGAUCUCUUGUAGAUCU |
20.3 | 16.7 |
| 3 | 36‐58 | UCUUGUAGAUCUGUUCUCUAAAC |
UUAGAGAACAGAUCUACAAGA UUGUAGAUCUGUUCUCUAAAC |
20.2 | 20.3 |
| 4 | 38‐60 | UUGUAGAUCUGUUCUCUAAACGA |
GUUUAGAGAACAGAUCUACAA GUAGAUCUGUUCUCUAAACGA |
11.7 | 20.2 |