TABLE 2.

Details of off target sequences for the best siRNA‐4

Strand (5′→3′)	No. of off targets	Complementary sequence (5′→3′)	Alignment of complementary sequence with off targets in humans a	Details of off target sequences (NCBI reference number or GenBank ID)
Guide GUUUAGAGAACAGAUCUACAA	0	TTGTAGATCTGTTCTCTAAAC	Nil	Nil
Passenger GUAGAUCUGUUCUCUAAACGA	7	CGTTTAGAGAACAGATCTA	CGTTTAGAGAACAGATCTA TATTTAGAAAACAGATCTA	NM_022757.3
			CGTTTAGAGAACAGATCTA TGTTTAGAAAACAGATCTCC	AK054685
			CGTTTAGAGAACAGATCTA AGTTTAGAGATCAGAGCTA	NM_176814.3
			CGTTTAGAGAACAGATCTA AGTTTAGAGACCAGATCAA	NM_018451.2
			CGTTTAGAGAACAGATCTA CTTTTTGAGAAAAGATCTA	BX647535
			CGTTTAGAGAACAGATCTA CATTTACAGAACAGCTCTA	NM_033087.1 NM_197973.1
			CGTTTAGAGAACAGATCTA CATTTAGAGAACACATTTA	NM_173645.1

^a Residues highlighted in red shows the mismatches with the passenger strand sequence