(A) Control, ndPTEN, p53-hKO, and ndPTEN::p53-hKO mice were subjected to intraperitoneal injection of 20% CCl4. At 2 days after CCl4 injection, cryosections of livers were analyzed by immunofluorescence microscopy using anti-PTEN antibodies. DAPI was used to stain nuclear DNA. (B) The intensity of PTEN signals in the nucleus was quantified relative to that in the cytosol. Bars are average ± SD (n = 161–221 hepatocytes from three mice). (C) Levels of p53 mRNA in the livers were quantified relative to those of albumin mRNA after CCl4 injection. Values were normalized to control mice without CCl4 treatment. Bars are average ± SD (n = 4 livers). One-way ANOVA with post-hoc Tukey was performed in (B and C): *p < 0.05, ***p < 0.001.