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. Author manuscript; available in PMC: 2022 Apr 16.
Published in final edited form as: Biochem Biophys Res Commun. 2021 Mar 2;549:83–90. doi: 10.1016/j.bbrc.2021.02.093

Figure 2. Oxidative stress induces DNA damage in ndPTEN mice, but not p53-hKO mice.

Figure 2.

(A) Paraffin sections of livers from the indicated mice were analyzed by immunohistochemistry using anti-phospho-H2AX (serine139) antibodies at 2 days after CCl4 injection. Nuclei were counterstained with hematoxylin. (B) Percentages of phospho-H2AX-positive cells were quantified. Bars are average ± SD (n = 3–6 livers). (C and D) Livers were isolated from the indicated mice after CCl4 injection and analyzed by Western blotting with antibodies to phospho-H2AX and GAPDH. (D) The intensity of phospho-H2AX signals was quantified relative to that of GAPDH. Bars are average ± SD (n = 5 livers). (E) Cryosections of livers were analyzed by immunofluorescence microscopy using antibodies of CK19 and phospho-H2AX. One-way ANOVA with post-hoc Tukey was performed in (B and D): *p < 0.05.