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. Author manuscript; available in PMC: 2022 Apr 16.
Published in final edited form as: Biochem Biophys Res Commun. 2021 Mar 2;549:83–90. doi: 10.1016/j.bbrc.2021.02.093

Figure 4. Fibrosis is induced in CCA in p53-hKO and ndPTEN::p53-hKO mice.

Figure 4.

(A) Paraffin liver sections were subjected to Sirius Red staining. T: Tumors, N: Non-tumor regions. (B) Fibrotic regions were quantified based on Sirius Red staining in no-tumor (normal), HCC, and CCA regions. Bars are average ± SD (n = 3–5 livers). (C) Immunohistochemistry of active caspase-3. Nuclei were counterstained with hematoxylin. (D) Active caspase-3-positive cells were quantified. Bars are average ± SD (n = 3–5 livers). (E) Ki67 immunohistochemistry. Nuclei were counterstained with hematoxylin. (F) Ki67-positive cells were quantified. Bars are average ± SD (n = 3–5 livers). One-way ANOVA with post-hoc Tukey was performed in (B, D, and F): ns, not significant.