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Ligand dependent effects on the sodium bound states of A2aR. (A) Schematic illustrating the sodium binding pocket of A2aR in an inactive conformation (PDB 4EIY) and the collapse of the pocket upon adopting an active conformation (PDB 3QAK). 13+ charge state of A2aR electrosprayed from (B) 200 mM NH4OAc and (C) 50 mM NaCl, 5 mM Tris (both pH 7.5 with 2× CMC LMNG) in the absence or presence of 10 μM ligand. Spectra are representative of n ≥ 4 nanoemitters and 2 protein preparations.
