Table 1. Proteins identified with higher abundance in the uterine fluid of the Cyclic group using MALDI TOF/TOF and LC-MS/MS, with MASCOT and PEAKS software, and compared with Database Equidae / Uniprot.
| Spot a | Protein name | GENEb | Access number | Protein scorec | Coveraged | Theoretical/Experimental | Peptide sequence | Peptide scoree | |
|---|---|---|---|---|---|---|---|---|---|
| MW (kDa) | pI | ||||||||
| 70 | Uncharacterized protein | TF | F6ZEH8 | 156.12# | 11% | 109.1/71.0 | 8.39/8.0 | TEPQTHYYAVAVVK (*) | 36.38≈ |
| SKDFHLFSSPHGK (*) | 42.65≈ | ||||||||
| HCEFDKFFR (*) | 52.56≈ | ||||||||
| EGCAPGYR (*) | 32.74≈ | ||||||||
| YYGYTGAFR (**) | 97%‡ | ||||||||
| 88Ϯ | CLVEKGDVAFVK (*) | 32.75≈ | |||||||
| SGNFQLLCPDGTRK (*)Ϯ | 31.75≈ | ||||||||
| AVTEFESCNLAK (*)Ϯ | 55.38≈ | ||||||||
| APNHAVVSR (*) | 44.63≈ | ||||||||
| YLTAVANLR (*) | 46.49≈ | ||||||||
| 106 | Serum albumin | ALB | F7BAY6 | 226.52# | 39% | 68.3/61.0 | 5.78/6.59 | SEIAHR (*) | 43.87≈ |
| FNDLGEK (*) | 44.74≈ | ||||||||
| LVNEVTEFAKK (*) | 44.90≈ | ||||||||
| SLHTLFGDK (*) | 43.47≈ | ||||||||
| LCTVATLR (*) | 27.11≈ | ||||||||
| DDHPNLPK (*) | 26.39≈ | ||||||||
| 336Ϯ | YLYEVAR (***) | 54.18≈ | |||||||
| ADFTECCPADDKAGCLIPK (*) | 43.22≈ | ||||||||
| CSSFQNFGER (*) | 71.73≈ | ||||||||
| ECCHGDLLECADDRADLAK (*) | 42.15≈ | ||||||||
| YICEHQDSISGK (*) | 62.78≈ | ||||||||
| DVFLGTFLYEYSR (**) | 95%‡ | ||||||||
| RHPDYSVSLLLR (***) | 41.42≈ | ||||||||
| IAKTYEATLEK (*) | 44.51≈ | ||||||||
| KAPQVSTPTLVEIGR (*) | 65.06≈ | ||||||||
| LPESER (*) | 43.82≈ | ||||||||
| LPCSENHLALALNR (*) | 63.71≈ | ||||||||
| LCVLHEK (*) | 34.36≈ | ||||||||
| TPVSEK (*) | 49.51≈ | ||||||||
| RPCFSALELDEGYVPK (***) | 53.01≈ | ||||||||
| KQSALAELVK (*) | 38.35≈ | ||||||||
| TVLGNFSAFVAK (*) | 45.67≈ | ||||||||
| 130 | Immunoglobulin lambda light chain variable region | IGL | A0A0A1E6K7 | 87.25# | 11% | 23.1/23.0 | 7.61/5.8 | VNDAVTTDGVQTTR (*) | 65.93≈ |
| SYSSVSCQVK (*) | 27.03≈ | ||||||||
| Apolipoprotein A1 | APOA1 | F6Z2L5 | 234Ϯ | 11% | 30.3/23.0 | 5.65/5.8 | VAPLSDEFR (**) | 99%‡ | |
| VNLAPFSEELR (**) | 99%‡ | ||||||||
| AHPALEDLR (**) | 97%‡ | ||||||||
| 133 | Immunoglobulin lambda light chain variable region | IGL | A0A0A1E6K7 | 84.36# | 10% | 23.1/29.0 | 7.61/6.5 | VNDAVTTDGVQTTR (*) | 54.17≈ |
| YAASSYLTR (*) | 47.38≈ | ||||||||
| 162 | Uncharacterized protein | IGHCp* | H9GZT5 | 76.96# | 7% | 36.4/56.0 | 8.27/8.13 | VVSILAIQHK (*) | 41.71≈ |
| ALPAPVER (*) | 31.20≈ | ||||||||
| LTVETNR (*) | 35.92≈ | ||||||||
| 166 | Hemopexin | HPX | F6X1I8 | 101.93# | 7% | 51.3/62.0 | 7.58/6.7 | NFIGPADAAFR (*) | 43.79≈ |
| FNPVSGEVPPK (*) | 41.65≈ | ||||||||
| GGHTLVDGYPK (*) | 56.53≈ | ||||||||
| 291 | Apolipoprotein A1 | APOA1 | F6Z2L5 | 130.67# | 18% | 30.3/23.0 | 5.65/5.12 | EYVAQFEASALGK (*) | 59.21≈ |
| DTEGLRQELNK (*) | 43.92≈ | ||||||||
| 70Ϯ | VAPLSDEFREGAR (*) | 42.98≈ | |||||||
| VNLAPFSEELR (**) | 99%‡ | ||||||||
a
Spot ID corresponds to the numbers shown in Figure 2; bGene designation in UniProt. N/A Gene not characterized; cScore Protein of PEAKS(#) or MASCOT (Ϯ); dProtein coverage calculated (identified amino acids/total amino acids); ePeptide score data obtain of PEAKS (P-value of the probability of correspondence ≈) or SCAFFOLD (sequence probability percentage ‡). MW – Molecular Weight; pI - Isoelectric point. (*) Acquisition of mass spectra of the peptide identified by LC-MS / MS; (**) Acquisition of mass spectra of the peptide identified by MALDI TOF/TOF; (***) Acquisition of mass spectra of the peptide identified by both LC-MS/MS and MALDI TOF/TOF.