Table 2. Proteins identified with higher abundance in the uterine fluid of the Fragment group using MALDI TOF/TOF and LC-MS/MS, with MASCOT and PEAKS software, and compared with Database Equidae / Uniprot.
| Spot a | Protein name | GENEb | Access number | Protein scorec | Coveraged | Theoretical/Experimental | Peptide sequence | Peptide scoree | |
|---|---|---|---|---|---|---|---|---|---|
| MW (kDa) | pI | ||||||||
| 32 | Leukocyte elastase inhibitor | SERPINB1 | P05619 | 79.39# | 16% | 43.0 /39 | 5.78/5.68 | ADLSGMSGAR (*) | 54.12≈ |
| LGVQDLFNR (*) | 50.55≈ | ||||||||
| VLELPYQGK (*) | 48.81≈ | ||||||||
| 196Ϯ | IPELLVK(*) | 34.25≈ | |||||||
| ALYFDTVEDIHSR (**) | 98%‡ | ||||||||
| HNPSANILFLGR (**) | 100%‡ | ||||||||
| 33 | Fibrinogen beta chain | FGB | F6PH38 | 71.57# | 10% | 55.7/40.0 | 8.63/5.5 | ALYEGFTVK (***) | 41.50≈ |
| QGFGNIATNADGK (*) | 28.89≈ | ||||||||
| 185Ϯ | IRPYFPQQ (*) | 27.64≈ | |||||||
| QDGSVDFGR (**) | 92%‡ | ||||||||
| EDGGGWWYNR (**) | 97%‡ | ||||||||
| 48 | Uncharacterized protein | IGHCp* | H9GZT5 | 91.65# | 19% | 36.4/47.0 | 8.27/7.5 | VVSILAIQHK (*) | 47.13≈ |
| DVLMISR (*) | 37.62≈ | ||||||||
| SQTYICNVAHPASSTK (*) | 29.70≈ | ||||||||
| 129Ϯ | VSVTCLVK (*) | 28.74≈ | |||||||
| LTVETNR (*) | 27.43≈ | ||||||||
| EPQVYVLAPHRDELSK (**) | 99%‡ | ||||||||
| Immunoglobulin gamma 1 heavy chain constant region | IGHC1 | Q95M34 | 97.46# | 12% | 37.4/47.0 | 7.68/7.5 | FNWYMDGVEVR (*) | 55.97≈ | |
| IQHQDWLSGK (*) | 39.67≈ | ||||||||
| VNNQALPQPIER (*) | 42.14≈ | ||||||||
| VSVTCLVK (*) | 28.74≈ | ||||||||
| 73 | Serotransferrrin | TF | P27425 | 211.04# | 24% | 78.0/71.0 | 6.83/7.7 | SIVPAPPLVACVK (*) | 55.82≈ |
| RTSYLECIK (*) | 38.76≈ | ||||||||
| KNSNFQLNQLQGK (*) | 44.23≈ | ||||||||
| CLADGAGDVAFVK (*) | 70.04≈ | ||||||||
| SKDFHLFSSPHGK (*) | 54.06≈ | ||||||||
| DSALGFLR (*) | 45.89≈ | ||||||||
| SSSDPDLTWNSLK (*) | 55.81≈ | ||||||||
| HCEFDKFFR (*) | 55.43≈ | ||||||||
| 76Ϯ | |||||||||
| YYGYTGAFR (***) | 51.49≈ | ||||||||
| CLVEKGDVAFVK (*) | 59.20≈ | ||||||||
| HQTVEQNTDGRNPDDWAK (*) | 54.48≈ | ||||||||
| SCYLAR (*)Ϯ | 38.27≈ | ||||||||
| AACVCQELHNQQASYGK (*)Ϯ | 57.02≈ | ||||||||
| YLTAVANLR (*) | 52.77≈ | ||||||||
| LLEACTFHRV (*)Ϯ | 50.24≈ | ||||||||
| 107 | Serum albumin | ALB | F7BAY6 | 247.80# | 50% | 68.3/58.0 | 5.78/6.3 | DTHKSEIAHR (*) | 53.72≈ |
| FNDLGEK (*) | 25.96≈ | ||||||||
| LVNEVTEFAKK (*) | 38.01≈ | ||||||||
| CAADESAENCDK (*) | 66.75≈ | ||||||||
| SLHTLFGDKLCTVATLR (*) | 61.95≈ | ||||||||
| ATYGELADCCEK (*) | 48.10≈ | ||||||||
| DDHPNLPK (*) | 46.72≈ | ||||||||
| ADFTECCPADDKAGCLIPK (*) | 31.66≈ | ||||||||
| LDALKER (*) | 34.17≈ | ||||||||
| LSQKFPK (*) | 39.65≈ | ||||||||
| ADFAEVSKIVTDLTK (*) | 47.17≈ | ||||||||
| ECCHGDLLECADDRADLAK (*) | 28.69≈ | ||||||||
| YICEHQDSISGK (*) | 43.12≈ | ||||||||
| ACCDKPLLQK (*) | 43.84≈ | ||||||||
| SHCIAEVK (*) | 43.75≈ | ||||||||
| DAKDVFLGTFLYEYSR (*) | 42.59≈ | ||||||||
| RHPDYSVSLLLR (***) | 58.66≈ | ||||||||
| IAKTYEATLEK (*) | 41.89≈ | ||||||||
| KAPQVSTPTLVEIGR (*) | 66.02≈ | ||||||||
| TLGKVGSR (*) | 42.99≈ | ||||||||
| LPESERLPCSENHLALALNR (*) | 48.49≈ | ||||||||
| LCVLHEKTPVSEK (*) | 54.06≈ | ||||||||
| CCTDSLAER (*) | 43.70≈ | ||||||||
| RPCFSALELDEGYVPK (***) | 48.74≈ | ||||||||
| KQSALAELVK (*) | 34.81≈ | ||||||||
| ATKEQLK (*) | 36.04≈ | ||||||||
| TVLGNFSAFVAK (*) | 60.22≈ | ||||||||
| CCGAEDKEACFAEEGPK (*) | 60.69≈ | ||||||||
| LVASSQLALA (*) | 38.07≈ | ||||||||
| 149 | Uncharacterized protein | CFB | F6RMD0 | 76.57# | 3% | 85.9/89.0 | 6.75/7.03 | LEDSVTYYCSR (*) | 30.83≈ |
| DISAVVTPR (*) | 61.15≈ | ||||||||
| 173 | Uncharacterized protein | IGHCp* | H9GZT5 | 154.02# | 24% | 36.4/47.0 | 8.27/7.79 | SQTYICNVAHPASSTK (*) | 69.58≈ |
| DVLMISR (*) | 44.61≈ | ||||||||
| VVSILAIQHK (*) | 57.55≈ | ||||||||
| TISKPTGQPR (*) | 58.01≈ | ||||||||
| 67Ϯ | EPQVYVLAPHR (**) | 95%‡ | |||||||
| VSVTCLVK (*) | 46.80≈ | ||||||||
| DFYPTDIDIEWK (*) | 43.58≈ | ||||||||
| LTVETNR (*) | 40.49≈ | ||||||||
| Immunoglobulin gamma 1 heavy chain constant region | IGHC1 | Q95M34 | 97.01# | 11% | 37.4/47.0 | 7.68/7.79 | IQHQDWLSGK (*) | 53.81≈ | |
| SQEPQVYVLAPHPDELSK (*) | 42.66≈ | ||||||||
| VSVTCLVK (*) | 46.80≈ | ||||||||
| 181 | Serotransferrin | TF | P27425 | 178.76# | 11% | 78.0/72.0 | 6.83/7.62 | RTSYLECIK (*) | 33.82≈ |
| TEPQTHYYAVAVVK (*) | 70.57≈ | ||||||||
| SKDFHLFSSPHGK (*) | 52.55≈ | ||||||||
| YYGYTGAFR (***) | 55.17≈ | ||||||||
| 147Ϯ | SCYLAR (*)Ϯ | 39.43≈ | |||||||
| APNHAVVSR (*) | 47.29≈ | ||||||||
| YLTAVANLR (*) | 40.12≈ | ||||||||
| LLEACTFHR (*)Ϯ | 48.50≈ | ||||||||
| 184 | Fibrinogen gamma chain | FGG | F6W2Y1 | 241Ϯ | 8% | 49.7/75.0 | 5.41/7.16 | YEALVVTHESTIR (**) | 99%‡ |
| IHLISTQTTIPYVLR (**) | 99%‡ | ||||||||
| VGPENDKYR (**) | 98%‡ | ||||||||
| 272 | Serotransferrin | TF | P27425 | 118Ϯ | 3% | 78.0/73.0 | 6.83/7.7 | YYGYTGAFR (**) | 93%‡ |
| APNHAVVSR (**) | 92%‡ | ||||||||
| 273 | Fibrinogen gamma chain | FGG | F6W2Y1 | 124.40# | 12% | 49.7/74.0 | 5.41/7.49 | YEALVVTHESTIR (*) | 74.60≈ |
| VAQLEAK (*) | 34.80≈ | ||||||||
| LDGSVDFKK (*) | 32.32≈ | ||||||||
| IHLISTQTTIPYVLR (**) | 96% ‡ | ||||||||
| VQLEDWNGK (*) | 49.87≈ | ||||||||
| Serotransferrin | TF | P27425 | 70.76# | 2% | 78.0/74.0 | 6.83/7.49 | DSALGFLR (*) | 35.62≈ | |
| LLEACTFHR (*) | 47.13≈ | ||||||||
| 377 | Lipocalin 2 | LCN2 | F6TIR2 | 280Ϯ | 17% | 23.1/20.0 | 5.94/5.8 | LIPAPPLDR (**) | 98%‡ |
| YFGVQSYIVR (**) | 100% ‡ | ||||||||
| VADTDYNQFAIVFFR (**) | 100%‡ | ||||||||
| 378 | Lipocalin 2 | LCN2 | F6TIR2 | 154.20# | 44% | 23.1/20.0 | 5.94/6.65 | LIPAPPLDR (**) | 99%‡ |
| VPLQPDFKDDQFQGK (*) | 57.29≈ | ||||||||
| KEEQGQFTMYTTTYELK (*) | 48.29≈ | ||||||||
| DQNCDHWIR (**) | 97%‡ | ||||||||
| 326Ϯ | YFGVQSYIVR (***) | 63.22≈ | |||||||
| NQEYFKTTLYR (*) | 41.76≈ | ||||||||
| TKELTPELR (**) | 94%‡ | ||||||||
| EKFISFAK (***) | 41.10≈ | ||||||||
a
Spot ID corresponds to the numbers shown in Figure 2; bGene designation in UniProt or Abbreviation. *Abbreviation of the protein without gene identification; cScore Protein of PEAKS(#) or MASCOT (Ϯ); dProtein coverage calculated (identified amino acids/total amino acids); ePeptide score data obtain of PEAKS (P-value of the probability of correspondence ≈) or SCAFFOLD (sequence probability percentage ‡). MW – Molecular Weight; pI - Isoelectric point. (*) Acquisition of mass spectra of the peptide identified by LC-MS / MS; (**) Acquisition of mass spectra of the peptide identified by MALDI TOF/TOF; (***) Acquisition of mass spectra of the peptide identified by both LC-MS/MS and MALDI TOF/TOF.