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. 2021 Mar 23;20:15347354211002647. doi: 10.1177/15347354211002647

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — GT treatment on BM cells and biopsies of elderly AML-MRC patients. (A) ROS levels in BM CD34⁺ cells expressed as MFI were analyzed by flow cytometry. (B) Representative image of NRF2 expression in BM CD34⁺ cells analyzed by immunofluorescence microscopy. Images were collected using 63× optical zoom. (C) Representative image of NRF2 and HIF-1α protein expression in BM biopsy specimens analyzed before by immunohistochemistry using a 40× objective. BM samples of 10 AML-MRC patients were analyzed before and after 30 days of treatment.

Abbreviations: AML-MRC, acute myeloid leukemia with myelodysplasia-related changes; BM, bone marrow; GT, green tea; MFI, mean fluorescence intensity; ROS, reactive oxygen species.