Figure 5.

KDM3A functioned as an oncogene in NSCLC by inhibiting p21

(A) Western blots and quantification of KDM3A, p53-k372me1, and p53 in H1299 cells, normalized to β-actin expression. (B) p21 was immunoprecipitated using p53 antibody relative to IgG by ChIP assays. (C) Western blots and quantification of p21, cleaved caspase-3, and caspase-3 in H1299 cells, normalized to β-actin expression. (D) H1299 cell viability was measured by CCK-8 assays. (E) Numbers of colonies derived from H1299 cells. (F) H1299 cell apoptosis determined by annexin V/PI-labeled flow cytometric analysis. (G) H1299 cell cycle determined by flow cytometric analysis. ∗p < 0.05 (compared with scramble siRNA or IgG antibody) and ^#p < 0.05 (compared with si-KDM3A or anti-p53 with si-NC) by unpaired t test, Tukey’s test-corrected one-way ANOVA, or Bonferroni-corrected repeated-measures ANOVA.