Figure 6.

lncRNA SNHG4 promoted the tumorigenicity of human NSCLC cells in vivo by increasing KDM3A and p21 expression

(A) Verification of lncRNA SNHG4 and/or p21 knockdown by qRT-PCR. (B) Western blots and quantification of KDM3A, p21, cleaved caspase-3, and caspase-3 in H1299 cells, normalized to β-actin expression. (C) The growth of mouse xenotransplanted tumors of H1299 cells at indicated time points. (D) Weight of mouse xenotransplanted tumors of H1299 cells. (E) Ki67-positive cells detected by immunohistochemical staining. (F) Cell apoptosis detected by TUNEL staining. (G) lncRNA SNHG4 and p21 expression detected by qRT-PCR. (H) Western blot analysis of KDM3A, p21, cleaved caspase-3, and caspase-3 in tumor tissues, normalized to β-actin expression. ∗p < 0.05 (compared with mice treated with scramble shRNA) and ^#p < 0.05 (compared with mice treated with lncRNA SNHG4-specific shRNA) by Tukey’s test-corrected one-way ANOVA or Bonferroni-corrected repeated-measures ANOVA.