Figure 2.

Microcontainer organoids show hallmarks of structure and composition

(A) Organoid lumenization shown via orthogonal projection. Arrowheads denote secondary chamber within lumen.

(B) Flow cytometry plot showing sorted luminal and myoepithelial populations.

(C) Immunostaining showing bilayered organization of K14+ and K18 + cells. Organoids in the top row used cells from a 66-year-old specimen; organoids in the bottom row were from a different experiment using cells from a 19-year-old specimen.

(D) Immunostaining showing basal polarization of integrin. Zoomed inset at right. Scale bars are 50 μm.