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3′ reversible terminator protecting groups. (A) General structure for a 3′ O-triphosphate nucleotide, where N is any one of the nucleobases shown in Figure 1B. (B) Methyl, (C) 2-nitrobenzyl, (D) 3′-O-(2-cyanoethyl), (E) allyl, (F) amine, (G) azidomethyl, and (H) tert-butoxy ethoxy (TBE).
