. 2021 Mar 2;24(4):102259. doi: 10.1016/j.isci.2021.102259

Table 1.

List of cleavage sites grouped into the metalloprotease cluster

UniProt acc.^a	Gene name^a	Protein name^a	Cleavage window^a	P1^a	ADAM10 PWM score^b	ADAM17 PWM score^b
P55283	CDH4	Cadherin-4	TIGA↓VAAA	724	5.10	8.23
Q9H3T3	SEMA6B	Semaphorin-6B	GPGR↓LTPA	55	3.64	2.35
P04156	PRNP	Major prion protein	GAAA↓AGAV	117	4.54	5.51
P04156	PRNP	Major prion protein	AAAG↓AVVG	119	3.74	4.51
P04156	PRNP	Major prion protein	AGAA↓AAGA	116	4.24	4.67
P04156	PRNP	Major prion protein	AAGA↓VVGG	120	6.91	9.05
P55291	CDH15	Cadherin-15	GAAA↓LLAG	594	8.07	6.95
Q96NY8	PVRL4	Nectin-4	DPQE↓DSGK	337	−6.80	−4.09
P18827	SDC1	Syndecan-1	GPKE↓GEAV	101	−6.35	−5.73
P98172	EFNB1	Ephrin-B1	GASG↓GSSG	224	−0.33	−2.67
P10586	PTPRF	Receptor-type tyrosine-protein phosphatase F	GPFQ↓EVDG	364	−4.41	−2.74
Q13308	PTK7	Inactive tyrosine-protein kinase 7	VPEE↓SEGP	689	−6.23	−1.29
Q13308	PTK7	Inactive tyrosine-protein kinase 7	HPAS↓EAEI	136	−6.89	−4.66
P30466	HLA-B	HLA class I histocompatibility antigen, B-18 alpha chain	ISVG↓YVDG	50	2.44	−0.77
P05067	APP	Amyloid beta A4 protein	HHQK↓LVFF	687	6.61	1.80
P22223	CDH3	Cadherin-3	EVQR↓LTVT	348	2.66	3.67
P0C7U0	ELFN1	Protein ELFN1	YAAE↓VVGP	258	2.81	7.02
P09603	CSF1	Macrophage colony stimulating factor 1	KAFL↓LVQD	87	5.31	4.01
P04156	PRNP	Major prion protein	HMAG↓AAAA	114	3.98	5.00
Q5ZPR3	CD276	CD276 antigen	VSLQ↓VAAP	137	3.27	5.51
Q8WVN6	SECTM1	Secreted and transmembrane protein 1	GAEP↓QSAP	138	1.12	−0.59
P05067	APP	Amyloid beta A4 protein	VLAN↓MISE	579	2.09	0.35
O60462	NRP2	Neuropilin-2	PISA↓FAGE	806	3.02	2.37
P29317	EPHA2	Ephrin type-A receptor 2	QVQA↓LTQE	509	3.96	3.43
Q13308	PTK7	Inactive tyrosine-protein kinase 7	TPAG↓SIEA	488	2.16	1.41
Q9BRK3	MXRA8	Matrix-remodeling-associated protein 8	AAAG↓SSVV	33	1.45	1.31
P98172	EFNB1	Ephrin-B1	ASGG↓SSGD	225	0.27	−0.01
O75056	SDC3	Syndecan-3	GSSA↓AQLP	371	0.53	0.62
Q14126	DSG2	Desmoglein-2	VLEG↓MVEE	279	−2.71	−3.98
Q7Z5N4	SDK1	Protein sidekick-1	AVSA↓QVEA	1998	1.79	−1.62
P27824	CANX	Calnexin	VVGQ↓MIEA	472	0.93	−0.12
P16422	EPCAM	Epithelial cell adhesion molecule	DVAY↓YFEK	214	0.56	−1.84
P09758	TACSTD2	Tumor-associated calcium signal transducer 2	DAAY↓YFER	224	−0.05	−1.47
P55287	CDH11	Cadherin-11	NAEA↓YILN	608	2.10	3.07
P18827	SDC1	Syndecan-1	EGEA↓VVLP	104	−0.61	3.95
Q92823	NRCAM	Neuronal cell adhesion molecule	FVPY↓LIKV	817	2.38	3.18
Q9Y6N7	ROBO1	Roundabout homolog 1	VIPF↓LVPG	841	1.12	0.81
Q8N126	CADM3	Cell adhesion molecule 3	PTAM↓IRPD	236	1.10	2.31
P30480	HLA-B; HLA-A	HLA class I histocompatibility antigen, B-7 alpha chain	TLQS↓MYGC	121	−1.28	−1.22
P22223	CDH3	Cadherin-3	EPVC↓VYTA	460	−1.00	2.00
P04626	ERBB2	Receptor tyrosine-protein kinase erbB-2	TACP↓YNYL	300	2.36	−1.11
Q13740	ALCAM	CD166 antigen	EADE↓ISDE	513	−4.86	−1.57
Q9Y6N7	ROBO1	Roundabout homolog 1	PQPA↓IFWR	381	−2.50	−2.20
Q6YHK3	CD109	CD109 antigen	PSEA↓ISLS	1330	−1.14	1.18
Q08174	PCDH1	Protocadherin-1	NAEL↓VYSL	541	0.24	4.81
Q9Y624	F11R	Junctional adhesion molecule A	TSNA↓VRME	226	−1.04	0.15
P78504	JAG1	Protein jagged1	HPCY↓NSGT	792	1.70	−0.26
P10586	PTPRF	Receptor-type tyrosine-protein phosphatase F	QPNT↓EYSF	1071	−8.26	−4.91
P16070	CD44	CD44 antigen	DIYP↓SNPT	170	−3.85	−4.47
Q15262-2	PTPRK	Receptor-type tyrosine-protein phosphatase kappa	PDPA↓KQTD	745	−5.87	−4.31
Q92896	GLG1	Golgi apparatus protein 1	SDLA↓MQVM	1135	−3.57	−1.88
Q13308	PTK7	Inactive tyrosine-protein kinase 7	NSCN↓IKHT	671	−6.94	−1.51
P98155	VLDLR	Very low-density lipoprotein receptor	ICIN↓LKGG	412	−0.49	−2.39
Q92823	NRCAM	Neuronal cell adhesion molecule	LIIN↓IMSE	107	−2.44	−3.91
Q92823	NRCAM	Neuronal cell adhesion molecule	APQN↓LVLS	369	2.19	1.59
Q13332	PTPRS	Receptor-type tyrosine-protein phosphatase S	DPQP↓IVDG	1275	5.02	4.15
Q92823	NRCAM	Neuronal cell adhesion molecule	HPNG↓ILTE	980	0.61	0.51
P12830	CDH1	Cadherin-1	GAAG↓VCRK	693	−7.43	−0.27
P22223	CDH3	Cadherin-3	DPAG↓WLAM	484	−0.57	1.40
P18827	SDC1	Syndecan-1	ATGA↓SQGL	242	0.99	3.08
P30480	HLA-C	HLA class I histocompatibility antigen, Cw-7 alpha chain	DLRS↓WTAA	156	−3.91	1.61
Q14574	DSC3	Desmocollin-3	TPAA↓QYVR	475	2.20	0.76
Q14517	FAT1	Protocadherin Fat 1	PPFF↓FTIV	3477	2.92	2.73
P32926	DSG3	Desmoglein-3	TPMF↓LLSR	209	3.29	3.82
Q13740	ALCAM	CD166 antigen	NVSA↓ISIP	502	2.41	4.33
Q92896	GLG1	Golgi apparatus protein 1	LAMQ↓VMTS	1137	2.46	3.50
Q92823	NRCAM	Neuronal cell adhesion molecule	PAMA↓SRQV	1158	1.61	4.36
Q14210	LY6D	Lymphocyte antigen 6D	PSYT↓LQGQ	72	0.27	−0.23
P35613	BSG	Basigin	EPMG↓TANI	211	−0.01	2.09
P58658	EVA1C	Protein eva-1 homolog C	DPSG↓SKVL	294	−2.14	−0.98
P10586	PTPRF	Receptor-type tyrosine-protein phosphatase F	TPAQ↓QQEE	1254	−0.07	−4.15
Q12907	LMAN2	Vesicular integral-membrane protein VIP36	SVNF↓LKSP	298	3.04	3.67
P18827	SDC1	Syndecan-1	ASQG↓LLDR	245	0.55	−1.12
P35052	GPC1	Glypican-1	HPQL↓LLPD	182	3.64	0.73
Q14126	DSG2	Desmoglein-2	EIQF↓LISD	570	5.13	4.76
P15151	PVR	Poliovirus receptor	EVQK↓VQLT	153	1.30	3.26
Q14210	LY6D	Lymphocyte antigen 6D	LQGQ↓VSSG	76	0.94	3.05
P98172	EFNB1	Ephrin-B1	GPGA↓SGGS	222	1.06	2.28
P78504	JAG1	Protein jagged1	AVAE↓VRVQ	1054	−1.10	3.13
Q92823	NRCAM	Neuronal cell adhesion molecule	EVSG↓TQTT	702	−1.68	0.85
P05067	APP	Amyloid beta A4 protein	ELLP↓VNGE	608	1.35	3.06
Q92823	NRCAM	Neuronal cell adhesion molecule	TPEG↓VPSA	950	−0.12	3.10
P30508	HLA-C	HLA class I histocompatibility antigen, Cw-12 alpha chain	EPRF↓IAVG	46	0.07	4.02
P30480	HLA-C	HLA class I histocompatibility antigen, Cw-7 alpha chain	EPRF↓ISVG	46	−1.41	2.74
Q08174	PCDH1	Protocadherin-1	KKYF↓LQTT	457	−0.81	−2.64
P08581	MET	Hepatocyte growth factor receptor	GFMF↓LTDQ	228	6.58	1.36

The cleavage sites grouped into the metalloprotease cluster shown in Figure 6B are listed. The order from top to bottom is the same to that of the cluster.

Accessions, gene names, protein names, cleavage window sequences and P1 positions (the first upstream amino acid of the cleavage site) follow those in the SwissProt database utilized for database search. Cleavage sites are indicated with “↓”.

PWM scores for ADAM10 and ADAM17 shown in Figure 6B.