Table 1.
Whole cell recordings
| Whole cell parameters | |||||
|---|---|---|---|---|---|
| GlyR | Glycine | β-Alanine | Taurine | GABA | Imax glycine (nA) |
| Human α1 GlyR | |||||
| Irel | – | 0.73 ± 0.11 | 0.25 ± 0.09 | 0.017 ± 0.020 | 13.2 ± 3 |
| EC50 (μM) | 240 ± 60 | 860 ± 250 | 2,000 ± 400 | 61,000 ± 14,000 | |
| nH | 1.57 ± 0.22 | 1.5 ± 0.24 | 1.30 ± 0.11 | 1.32 ± 0.07 | |
| n | 10 | 6 | 7 | 5 | |
| Zebrafish α1 GlyR | |||||
| Irel | – | 0.84 ± 0.09 | 0.40 ± 0.20 | 0.14 ± 0.13 | 9.0 ± 1.4 |
| EC50 (μM) | 190 ± 60 | 340 ± 190 | 1050 ± 220 | 28,400 ± 3,000 | |
| nH | 1.9 ± 0.3 | 1.34 ± 0.19 | 1.26 ± 0.12 | 1.59 ± 0.17 | |
| n | 8 | 10 | 6 | 8 | |
| Human α1 GlyR ΔICD | |||||
| Irel | – | 0.85 ± 0.04 | 0.55 ± 0.07 | 0.07 ± 0.03 | 2.8 ± 1.0 |
| EC50 (μm) | 140 ± 20 | 450 ± 190 | 1500 ± 210 | 33,200 ± 11,000 | |
| nH | 1.43 ± 0.38 | 1.25 ± 0.24 | 1.05 ± 0.16 | 1.42 ± 0.17 | |
| n | 5 | 4 | 5 | 6 | |
| Human α1 GlyR ΔICD + TM4 zebrafish | |||||
| Irel | – | 0.86 ± 0.07 | 0.56 ± 0.06 | 0.10 ± 0.04 | 2.2 ± 0.6 |
| EC50 (μm) | 180 ± 32 | 320 ± 70 | 1480 ± 570 | 93,200 ± 45,000 | |
| nH | 1.19 ± 0.20 | 1.56 ± 0.24 | 1.03 ± 0.19 | 0.98 ± 0.11 | |
| n | 8 | 7 | 7 | 5 | |
| Zebrafish α1 GlyREM | |||||
| Irel | – | 0.93 ± 0.03 | 0.74 ± 0.13 | 0.62 ± 0.12 | 3.3 ± 0.8 |
| EC50 (μm) | 92 ± 10 | 170 ± 90 | 390 ± 140 | 12,400 ± 4000 | |
| nH | 1.6 ± 0.4 | 1.17 ± 0.15 | 1.03 ± 0.11 | 1.26 ± 0.22 | |
| n | 4 | 6 | 5 | 14 | |
Agonist efficacy and sensitivity values were estimated from the fit of dose-response curves and are presented as mean ± S.D. Irel, maximum current normalized to that of glycine in the same cell.