Table 1.

Solutions that can be prepared in advance and information about their treatment to achieve sterile and/or RNAse-free conditions

REAGENT	Volume	Sterilization method	RNAse-free
1 M Tris-HCl pH 6.8	1 L	Autoclave	DEPC water
1 M Tris-HCl pH 7.5	1 L	Autoclave	DEPC water
1 M Tris-HCl pH 9.5	1 L	Autoclave	DEPC water
0.5 M EDTA pH 8	100 mL	Autoclave	DEPC water
5 M NaCl	500 mL	Autoclave	DEPC water
1 M MgCl2	500 mL	n/a	n/a
50% Dextran Sulfate	50 mL	n/a	DEPC water
100 mg/mL tRNA	1 mL	n/a	DEPC water
10% Acetic Acid	20 mL	n/a	DEPC water
10× in situ salts	50 mL	Filter sterilize	DEPC water
20× SSC pH 7	1 L	n/a	DEPC water
10× PBS pH 7.4	1 L	Autoclave	n/a
1× PBS pH 7.4	1 L	n/a	n/a
50× TAE	1 L	Autoclave	n/a
1× TAE	1 L	n/a	n/a
1% (w/v) agarose in 1×TAE buffer	500 mL	n/a	n/a
50% Formamide	50 mL	n/a	DEPC water
Hybridization solution	40 mL	n/a	DEPC water
15, 30, 50, 70, 80, 90% ethanol	500 mL each	n/a	n/a
25, 50, 75, 100% Histo-Clear in ethanol	500 mL each	n/a	n/a
LB-Carbenicillin plates	n/a	n/a	n/a

Specific recipes for each reagent are found under “materials and equipment”.