Table 2.

Solutions that have to be prepared fresh and information about their treatment to achieve sterile and/or RNAse-free conditions

REAGENT	Volume	Sterilization method	RNAse-free
4% PFA-Triton in PBS pH 7.0	400 mL	n/a	n/a
4% PFA in PBS pH 7.0	400 mL	n/a	n/a
Proteinase K buffer pH 7.5 at 37°C	300 mL	n/a	n/a
0.2% Glycine solution	300 mL	n/a	n/a
0.1 M Triethanolamine	300 mL	n/a	n/a
0.2× SSC 12 to 14 h at 55°C (for day 2)	1 L	n/a	n/a
Block solution 1	50 mL	n/a	n/a
BSA block solution 2	300 mL	n/a	n/a
Buffer C	100 mL	n/a	n/a