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. 2021 Mar 10;41:101919. doi: 10.1016/j.redox.2021.101919

Fig. 2.

Fig. 2

ALDH2 knockout increases cholesterol synthesis. (A) Schematics of the major intermediates in pathways of de novo cholesterol synthesis. (B) LC-MS analysis of de novo cholesterol synthesis in WT and AKO mouse liver at 12th week (chow diet, n = 10). (C) 13C-acetate incorporation into cholesterol in WT and AKO hepatocytes (n = 3). (D) LC-MS analysis of intermediates in de novo cholesterol synthesis in 7702 cells transfected with ALDH2 siRNA (n = 3). (E) LC-MS analysis of cholesterol esters in 7702 cells transfected with ALDH2 siRNA (n = 3). (F–G) GC-MS analysis of free cholesterol in control and overALDH2 7702 cells treated with siCHC and lovastatin (n = 3). Statistical comparisons were made using a 2-tailed Student's t-test. All data are mean ± SD. *P < 0.05, **P < 0.01, WT: wild type; AKO: ALDH2 Knockout; CHC, Clathrin heavy chain.