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. 2021 Mar 10;41:101919. doi: 10.1016/j.redox.2021.101919

Fig. 3.

Fig. 3

ALDH2 destabilizes HMGCR through increasing its ubiquitination and proteasomal degradation in sterol-loaded cells. (A–B) HMGCR protein levels increased in AKO mouse hepatocytes compared to WT (n = 3). (C) HMGCR protein levels increased in ALDH2 knockdown 7702 cells (n = 3). (D–E) Representative western blot (D) and the quantification (E, n = 3) of HMGCR expression in 7702 cells transfected with different dose of flag-ALDH2 (WT) plasmids. (F–G) Representative western blot and the quantification of HMGCR expression in 7702 cells (treat with or without MG132) transfected with different dose of ALDH2 siRNA (n = 3). (H–I) The representative image and quantitation of ubiquitination of HMGCR in siALDH2 (H) and overexpressed ALDH2 7702 cells (I). (J–K) Representative western blot and the quantification of HMGCR expression in WT and AKO hepatocytes treated with lipoprotein deficient medium (LPDS) from 0 to 36 h (n = 3). Statistical comparisons were made using a 2-tailed Student's t-test or ANOVA. All data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.