Figure 6.

Schematic illustration of imaging data-processing

A median filter algorithm (1 px) was applied to raw images in order to remove sharp spike noises. For background subtraction, the mean intensity of the cell-free region was subtracted from each image. To remove the background and obtain the fluorescence intensity only from the target organelle, a mask image was created from an image in the TMR channel using the Otsu threshold algorithm and applied to images in both the TMR and ZnDA channels. Regions of interest (ROIs) were circled to select the target organelle within the single cell. The line profiles indicate the fluorescence intensities of HTL-TMR, which were measured along the green and magenta lines in the images.