Figure 5. Examples of lysosome- and Golgi-targeted probes.
(A) Superior LysoProbe, pH-sensitive rhodamine conjugated with N-linked glycan moieties for improved lysosomal targeting; Chen et al., 2015. (B) FIRE-pHLy, ratiometric pH sensor chimera with cyan fluorescent protein, mTFP1, and LAMP1 for targeting; Chin et al., 2020. (C) ChloropHore, DNA-based dual-ion reporter for lysosomal pH (FRET-induced) and intraluminal chloride; Leung et al., 2019. (D) Lysosomal-METRIQ, self-cleaving ratiometric probe for membrane trafficking and lysosomal integrity; Ishii et al., 2019. (E) L-cysteine carbon quantum dots (LC-CQD) with functionalized cysteines for Golgi anchoring; R. S. Li et al., 2017. (F) Rhodamine-sphingosine (RSG), pH sensor conjugated with lipid sphingosine for Golgi recognition; Fan et al., 2019. (G) Mnn2-pHluorin, green fluorescent protein pH sensor targeted to Golgi membranes; Deschamps et al., 2020. (H) Gol-SiRhoNox, Fe(II) sensor with myristoyl motif for Golgi targeting; Hirayama et al., 2019. Created with BioRender.com.