Fig. 2.

A 50% leucine restriction (LR) reduces fat mass by mainly inhibiting lipogenesis. a Direct images of subcutaneous white adipose tissue (sWAT), epididymal white adipose tissue (eWAT), and brown adipose tissue (BAT). b Hematoxylin and eosin (H&E) staining of adipocytes in sWAT (top), eWAT (middle), and BAT (bottom). c Western blotting and quantification of the protein levels of p-HSL, HSL, p-PLIN1, PLIN1 and ATGL in sWAT in 0% LR (control) or 50% LR. A.U.: arbitrary unit. d Relative mRNA expression levels of genes involved in lipogenesis, uptake, lipolysis and β-oxidation were measured. All mice were fed with a 0% LR (control) or 50% LR diet. Data are expressed as the mean ± SEM (n = 4–6 per group, as indicated), with individual data points. *p < 0.05