Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 26;40:113. doi: 10.1186/s13046-021-01904-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Knockdown of Elafin inhibits epithelial-mesenchymal transition (EMT) and metastasis of HCC cells in vitro and in vivo. a The efficiency of Elafin knockdown were measured by real-time PCR (top) and Western blotting (bottom) assays. *** P < 0.001. b Knockdown of Elafin resulted in suppressing migration of PLC-8024 and Huh7 cells. Scale bar, 100 μm. Statistical results are presented as mean ± SD (from triplicates), and significance is determined by Student t test (*** P < 0.001). c Knockdown of Elafin resulted in suppressing invasion of PLC-8024 and Huh7 cells. Scale bar, 100 μm. Statistical results are presented as mean ± SD (from triplicates) (*** P < 0.001). d Knockdown of Elafin impaired the scratch wound-healing ability of PLC-8024 and Huh7 cells. Scale bar, 100 μm. Statistical results at 48 h of scratch wound-healing assays are presented as mean ± SD (from triplicates) (** P < 0.01; *** P < 0.001). e Representative immunofluorescence images illustrating cytoskeleton of control and Elafin silencing cells. Scale bar, 20 μm. f Representative immunofluorescence images of EMT markers expression in Elafin silencing cells. Scale bar, 50 μm. g Expression of EMT markers mediated by Elafin silencing are shown by western boltting. h Down-regulation of Elafin significantly suppressed lung metastasis in nude mice model established by injection of indicated cells through the tail vein. Representative HE staining images are shown. Scale bar, 100 μm. i Lung metastasis nodules from the nude mice model are analyzed. *** P < 0.001