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. Author manuscript; available in PMC: 2022 Apr 10.
Published in final edited form as: Cancer Lett. 2021 Jan 17;503:231–239. doi: 10.1016/j.canlet.2021.01.012

Fig. 2. Tumors that develop in NRL-PRL/ ApcMin/+ females exhibit GSI-dependent cancer stem cell activity and elevated transcripts for Notch target genes.

Fig. 2.

(A) The frequency of CSC activity was assessed by the ability of tumor cells to form primary tumorspheres as described in the Methods (tumorspheres/ 10,000 cells). Mean ± S.E.M., N=3. (*, p<0.05; unpaired Student’s t test). (B) Ability to renew CSC activity was assessed by the ability of cells treated with DMSO, GSI, or iCRT14 (iCRT) during formation of primary tumorspheres to form secondary tumorspheres (tumorspheres/ 10,000 cells). Mean ± S.E.M., N=3. (*, p<0.05; **, p<0.01; one way ANOVA followed by Tukey Multiple Comparison post tests). (C) Relative levels of transcripts for Notch (i) and canonical Wnt (ii) target genes were determined by qRT-PCR. Mean ± S.E.M., N=3. (*, p<0.05; **, p<0.01, unpaired Student’s t test).