Table 1.
Diagnostic SNV markers for analyses of single AZFb-c amplicon deletions according to references [13–15]
| AZF region in Yq11 | AZF amplicon | Amplicon diagnostic SNV sites (A or B allele) | GenBank accession number | Diagnostic restriction enzyme | Position in Y-Chr. NC_000024.10,GRch38.p7 |
WALBAE* | BRA 1* | GI 2* |
|---|---|---|---|---|---|---|---|---|
| AZFb-c | b2 | b2_AZFc-SFV B | BV686548 | Mnl I | 22.512.862–22.513.511 | − | − | − |
| AZFb-c | g1 | g1_AZFc-SFV B | BV686551 | BsmA I | 22.968.940–22.968.441 | − | − | − |
| AZFb-c | g1 | g1_BPY2-1_SNV B | BV012732 | EcoRV | 23.004.292–23.004.757 | − | − | − |
| AZFb-c | r1 | DAZ-SNV_II A | G73163 | Mbo I | 23.169.401–23.170.051 | − | − | − |
| AZFb-c | r2 | DAZ -SNV_III A | G63907 | Taq I | 23.230.522–23.230.822 | − | − | − |
| AZFb-c | r2 | DAZ-SNV_IV A | G63908 | Alu I | 23.283.265–23.283.923 | − | − | − |
| AZFb-c | gr1 | Gr_AZFc-SFV A | BV686554 | SnaB I | 23.414.449–23.414.888 | − | − | − |
| AZFb-c | b3 | b3_AZFc-SFV B | BV686549 | Bln I | 23.693.459–23.693.968 | − | − | − |
| AZFc | yel1 | Goly_SNV_I B | BV012731 | Hha I | 24.209.050–24.209.580 | + | + | + |
| AZFc | g2 | g2_AZFc-SFV B | BV686552 | Tsp451 | 24.600.115–24.600.606 | + | + | + |
*Azoospermic men with “classical” genomic extension of AZFb deletion [8]. They display deletion of all AZFb-AZFc overlapping amplicons as expected after P5/proximal P1.2