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Down-regulation of the expression of E2F1 targets and inhibition of CDK2 activity in SARS-CoV-infected Vero E6 cells. Mock-infected (M) or SARS-CoV-infected (S) Vero E6 cells were formalin-fixed and heat-inactivated. Aliquots of total cell lysate was immunoblotted with cyclin E (first panel), CDK2 (second panel), P-p27 (third panel), anti-nucleocapsid (N)(fourth panel), and calnexin (fifth panel) antibody, respectively. Data are representative of two independent experiments.
