Figure 2.

Splicing can be modulated at multiple levels. Pre-splicing, modifying enzymes, like the methyltransferase PRMT5, can be inhibited preventing spliceosome assembly. Core spliceosome components, like SF3B1, can also be inhibited leading to unproductive splicing. Isoform modulation can also be a target of alternative splicing to switch to a less oncogenic protein isoform. Clinicaltrials.gov identifiers (NCT) are included where small molecule inhibitors or modulators are being tested in humans.