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. 2021 Mar 12;10:556654. doi: 10.3389/fcimb.2020.556654

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Copyright © 2021 Du, Huang, Wei, Mei, Long, Liao, Wan, Liu and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The S1-PFGE and Southern hybridization analysis of 3 strains hybrid resistance- and virulence-encoding plasmids. Notes: (A) S1 nuclease digestion of genomic DNA of K. pneumoniae strains was followed by PFGE. Plasmid bands are shown as linearized fragment on the gel. (B) Southern blot hybridization of bla _KPC-2 gene. Mark it with a red arrow. (C) Southern blot hybridization of the marker gene (rmpA2) of the virulence plasmid. Mark it with a blue arrow. Lane M, reference standard strain Salmonella serotype Braenderup H9812 restricted with Xbal. M, marker; S1-PFGE, S1 nuclease pulsed-field gel electrophoresis.