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. 2021 Mar 12;11:647870. doi: 10.3389/fcimb.2021.647870

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Copyright © 2021 Moitra, Basu, Pawlowic, Hsu and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion of L. major CEPT in the presence of pXNG4-CEPT. (A) Genomic DNA samples from WT, CEPT +/− clone #2, CEPT +/− +pXNG4-CEPT (clone #2), and cept¯ +pXNG4-CEPT (clones #2-3, 2-3, and 2-4) parasites were processed for Southern blot using radiolabeled probes for the ORF (top) or flanking sequence (bottom) of CEPT. Bands corresponding to chromosomal CEPT (top: 8252 bp, bottom: 3730 bp), pXNG4-CEPT (top: 3475 bp), PAC (bottom: 3076 bp) and BSD (bottom: 2876 bp) were indicated. (B) Promastigotes of WT, CEPT+/− +pXNG4-CEPT, and cept¯ +pXNG4-CEPT were cultivated at 27°C in complete M199 media and culture densities were determined using a hemocytometer. Error bars represent standard deviations from three experiments.