Figure 6.

CEPT is dispensable for L. major amastigotes. BALB/c mice were infected in the footpads with stationary phase promastigotes and treated with either GCV or PBS from day 1 to day 14 post infection as described in Materials and Methods. (A) Footpad lesions were recorded weekly. (B, C) Parasite numbers in the infected footpads were determined at the indicated times by limiting dilution assay (B) and qPCR (C). (D) The pXNG4-CEPT copy numbers in promastigotes and amastigotes (#/cell ± SDs) were determined by qPCR. The 9 weeks data for cept¯ +pXNG4-CEPT amastigotes were not available (NA) because the infected mice had reached the humane endpoint at 8 weeks. Error bars represent standard deviations (*p < 0.05, **p < 0.01, ***p < 0.001).