Table 5. Analysis of antigenic B-Cell epitope’s allergenicity and toxicity.
S. no | Protein name | Start | Epitope IEDB bepipred | Allergenicity | Toxicity | ABCpred | Start | Allergenicity | Toxicity |
---|---|---|---|---|---|---|---|---|---|
1 | ORF8 protein | 12 | QHQPYVVDDP | Allergenic | – | PYVVDDPCPIHFYSKW | 15 | Non-allergenic | |
2 | ORF7a protein | 58 | HVYQLRARSVSPKLFIRQE | Allergenic | – | HVYQLRARSVSPKLFI | 58 | Allergen | – |
3 | Membrane glycoprotein | 7 | TITVEELKK | Allergenic | – | ||||
4 | Envelope protein | 23 | YSRVKNLNSSRVP | Non-Allergenic | Non-toxic | YVYSRVKNLNSSRVPD | 21 | Allergen | – |
5 | ORF3a protein | 15 | LKQGEIKDATPSDFVR | Non-Allergenic | Non-toxic | QGEIKDATPSDFVRAT | 17 | Allergen | – |
IGTVTLKQGEIKDATP | 10 | Allergen | – | ||||||
6 | Surface glycoprotein | 55 | VSGTNGTKRF | Non-Allergenic | Non-toxic | HRSYLTPGDSSSGWTA | 230 | Non-allergenic | Non-toxic |
123 | DPFLGVYYHKNNKSWMESEFRVYSSA | Non-Allergenic | Non-toxic | TVEKGIYQTSNFRVQP | 292 | Allergen | – | ||
234 | LTPGDSSSGWTA | Non-Allergenic | Non-toxic | GCLIGAEHVNNSYECD | 633 | Non-allergenic | Toxic | ||
298 | YQTSNFRVQP | Allergenic | – | LQSYGFQPTNGVGYQP | 477 | Non-allergenic | Non-toxic | ||
315 | PNITNLCPFGEVFNATRFASVYAWNRKRISNC | Allergenic | – | EVRQIAPGQTGKIADY | 391 | Non-allergenic | Non-toxic | ||
389 | GDEVRQIAPGQTGKIAD | Non-Allergenic | Non-toxic | TPTWRVYSTGSNVFQT | 615 | Non-allergenic | Non-toxic | ||
641 | VNNSYECDIPI | Non-Allergenic | Non-toxic | VIGIVNNTVYDPLQPE | 1114 | Non-allergenic | Non-toxic | ||
680 | YTMSLGAENSVAYSNN | Non-Allergenic | Non-toxic | SQSIIAYTMSLGAENS | 674 | Non-allergenic | Non-toxic | ||
792 | PDPSKPSKR | Non-Allergenic | Non-toxic | VSGTNGTKRFDNPVLP | 55 | Allergen | – | ||
FPNITNLCPFGEVFNA | 314 | Allergen | – | ||||||
7 | Orf1ab polyprotein | 373 | CHNSEVGPEH | Allergenic | – | VVKIYCPACHNSEVGP | 365 | Non-allergenic | Non-toxic |
813 | FTLKGGAP | Non-Allergenic | Non-toxic | TLKGGAPTKVTFGDDT | 814 | Allergen | – | ||
1482 | AVTAYNGYLTSSSKTPEE | Non-Allergenic | Non-toxic | TSRYWEPEFYEAMYTP | 3996 | Allergen | – | ||
3072 | GCSCDQLREPMLQSADAQS | Non-Allergenic | Non-toxic | SEAVEAPLVGTPVCIN | 771 | Non-allergenic | Non-toxic | ||
SSEIIGYKAIDGGVTR | 2242 | Non-allergenic | Non-toxic |