Fig. 2.

Development of a confluent endothelium in the vessel using the graded flow protocol in various co-culture conditions. (a) Progression of endothelium alignment and confluence throughout the flow protocol for the TIME only platform. The 0 hour time point, imaged after channel formation, initiated with TIME cells in a rounded morphology. The subsequent 48 hour of flow promoted TIME cell spreading and proliferation followed by alignment of the TIME cells in the direction of flow. The resulting confluent endothelium at 78 hours serves to function as a barrier for transendothelial flow; scale bar: 200 μm. (b) The resulting in vitro vascularized breast tumor platforms consisting of monoculture of TIME cell seeded lumen (red) or co-culture of GFP labeled (green) MDA-IBC3, SUM149, and MDA-MB-231 tumor cells around a TIME cell seeded lumen (red); scale bar: 500 μm.