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Independent variables and responses used in a 23 full factorial experimental design for the formulation and optimization of SIT-GA nano-conjugates.
| Independent Variables | Levels | |
|---|---|---|
| (‒1) | (+1) | |
| X1: SIT concentration (mM) | 1 | 10 |
| X2: GA concentration (mM) | 1 | 10 |
| X3: pH | 6 | 10 |
| Responses | Desirability constraints | |
| Y1: particle size (nm) | Minimize | |
| Y2: zeta potential (mV) | Maximize | |
Abbreviations: SIT = sitagliptin; G.A., glatiramer acetate.
