Figure 1.

Phylogenetic analysis of APX proteins from Populus trichocarpa, Arabidopsis thaliana, and Oryza sativa. A multiple alignment of the full-length amino acid sequences was generated using Clustal X 2.0. The unrooted phylogenetic tree was constructed using the neighbor-joining method with 1000 bootstrap replicates using MEGA 7.0. Different species are represented by different colors. The APX proteins are classified into three clades (I, II, and III) based on their subcellular localization.