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In silico analysis of the three RT-qPCR for TOSV detection included in the Trio TOSV RT-qPCR assay. In blue, TOSV strains; in yellow, viruses belonging to Sandfly Fever Naples virus serocomplex; in green, viruses belonging to Salehabad virus serocomplex; in orange, Sandfly Fever Sicilian virus serocomplex. TOSV genetic lineage (A–C) is indicated after the symbol “_” in the strain’s name.
