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Diurnal variability of serum EV particle abundance (A), size (B), total protein concentration (C) and expression of EV-associated protein markers (D–H), quantified by nanoparticle tracking analysis (NTA), microBCA assay and liquid chromatography mass spectrometry (LCMS) in the morning (AM) and afternoon (PM) on study days 1 and 3 in healthy volunteers (n = 20). Statistical analysis performed using paired t-tests. * p ≤ 0.05, ** p ≤ 0.01.
