. 2021 Feb 24;10(3):485. doi: 10.3390/cells10030485

Table 5.

Pooled analysis of EVs collected from serum of healthy female (n = 10) and male (n = 10) volunteers on study days 1 and 3 in the morning and afternoon. Data presented as geometric mean and 95% confidence interval (CI) of particle count, mode size, total protein concentration, and EV-associated protein marker abundance. Statistical analysis performed using one-way ANOVA. ns: not significant, * p ≤ 0.05, *** p ≤ 0.001, **** p ≤ 0.0001.

	Particle Count (Particles/mL)
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	7.41 × 10¹⁰	7.76 × 10¹¹	****	<0.0001	1.10 × 10¹¹	5.01 × 10¹¹	***	0.0002
95% CI Lower	3.47 × 10¹⁰	5.62 × 10¹¹			6.03 × 10¹⁰	3.24 × 10¹¹
95% CI Upper	1.58 × 10¹¹	1.10 × 10¹²			1.95 × 10¹¹	7.94 × 10¹¹
	Mode Size (nm)
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	88.8	86.0	ns	0.610	83.9	81.3	ns	0.622
95% CI Lower	77.8	76.5			79.2	75.8
95% CI Upper	101.4	96.8			88.8	87.2
	Protein Concentration (µg/mL)
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	330.4	865.0	*	0.037	289.1	509.3	ns	0.822
95% CI Lower	154.5	335.0			97.5	358.1
95% CI Upper	706.3	2238.7			855.1	722.8
	ASGR1 Response
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	136.5	183.2	ns	0.801	248.9	321.4	ns	0.844
95% CI Lower	70.2	110.4			94.6	129.1
95% CI Upper	265.5	304.1			654.6	799.8
	CD9 Response
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	70.6	73.5	ns	0.987	56.8	49.3	ns	0.837
95% CI Lower	46.7	49.8			32.1	37.8
95% CI Upper	106.9	108.4			100.7	64.4
	CD63 Response
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	29.6	39.5	ns	0.964	57.9	78.9	ns	0.959
95% CI Lower	5.1	5.3			6.5	15.2
95% CI Upper	171.0	293.1			515.2	408.3
	CD81 Response
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	103.50	135.8	ns	0.641	73.0	71.9	ns	0.999
95% CI Lower	69.2	87.5			35.4	48.4
95% CI Upper	155.2	210.9			150.3	107.2
	TSG101 Response
	AM				PM
	Female	Male	Difference	p	Female	Male	Difference	p
Mean	8.0	9.0	ns	0.945	11.7	10.5	ns	0.944
95% CI Lower	5.3	4.6			5.7	6.6
95% CI Upper	12.1	17.6			24.2	16.7