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. 2021 Feb 24;10(3):255. doi: 10.3390/pathogens10030255

Figure 1.

Figure 1

Phylogenetic relationships among Giardia duodenalis assemblages and sub-assemblages identified in infected symptomatic and asymptomatic children in the Zambézia province, Mozambique. The analysis was conducted by a neighbor-joining method of a 412-bp fragment (corresponding to position 79–490 of reference sequence L40508) of the gdh gene sequence. Genetic distances were calculated using the Kimura two-parameter model. Green filled squares represent sequences generated in the present study. Purple filled dots represent reference sequences. Bootstrap values lower than 50% are not displayed. Giardia ardeae was used as outgroup taxon to root the tree.