Figure A2.

HPLC Separation of 2Cl-adenine and 2Cl-adenosine nucleotides: (A) UV spectra of the chromatographic separations of the reactions with APT alone (APT), APT and PPK (APT/PPK), the adenylate kinase reaction (Adenylate kin.), and the full cascade with all three enzymes (APT/PPK/RNR). 2Cl-adenine and the 2Cl-adenosine (deoxy)ribonucleotides show a specific absorption maximum at 264 nm. (B) Chromatograms of the reactions with APT alone (APT), APT and PPK (APT/PPK), the adenylate kinase reaction (Adenylate kin.), and the full cascade with all three enzymes at a wavelength of 260 nm. The peaks at 17 and 18 min are the reducing agent DTT and the effector dGTP, respectively.