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. 2021 Feb 25;11(3):346. doi: 10.3390/biom11030346

Table 2.

Kinetic parameters of the enzymes: The kinetic parameters KM and kcat were determined for each enzyme and reaction step. Since the PPK catalyzes two reaction steps, the kinetic parameters were determined for each separately.

Enzyme Substrate KM, [µmol L−1] kcat, [s−1]
APT adenine 11.8 ± 5.2 119 ± 14
PPK AMP 41 ± 6.3 17 ± 1.3
PPK ADP 144 ± 14 0.43 ± 0.02
RNR ATP 199 ± 13 0.56 ± 0.02