Table 1.
Conditions for MPLC separation of metabolites and radioactivity of chromatographic fractions of the Rhodococcus sp. P1Y culture after incubation for 2 h in the presence of 3H-labeled ABA.
| No. of Fraction | Starting Solvents Ratio, Vol % | Final Solvents Ratio, Vol % | Radioactivity, cpm µL−1 |
|---|---|---|---|
| 1 | A 100 | A-B 90:10 | 10.1 1 |
| 2 | A-B 90:10 | A-B 80:20 | 7.0 |
| 3 | A-B 80:20 | A-B 70:30 | 2.2 |
| 4 | A-B 70:30 | A-B 60:40 | 2.5 |
| 5 | A-B 60:40 | A-B 50:50 | 1.0 |
| 6 | A-B 50:50 | A-B 40:60 | - |
| 7 | A-B 40:60 | A-B 30:70 | - |
| 8 | A-B 30:70 | A-B 20:80 | - |
| 9 | A-B 20:80 | A-B 10:90 | 3.5 |
| 10 | A-B 10:90 | B 100 | 34.5 |
| 11 | B 100 | B-C 50:50 | 168.2 |
| 12 | B-C 50:50 | C 100 | 164.3 |
| 13 | C 100 | C 100 | 5.6 |
1 The volume of each fraction was 45 mL.