Figure 2.

Enhanced immunoreactivity of ECM glycoproteins in the glaucomatous HET retina. Most representative immunohistochemical ECM stainings are shown. Fibronectin (red; (A,B)) and laminin (green; (D,E)) signals were found in blood vessels. A prominent laminin immunoreactivity was also found in the INL, GCL, and ILM. Staining of tenascin-C (red; (G,H)) and tenascin-R (green; (J,K)) was localized to the plexiform layers. The immunoreactivity of the glycoproteins fibro-nectin, laminin and tenascin-C was significantly upregulated in HET retinae (C,F,I). A comparable tenascin-R signal could be detected in HET and WT retinae (L). Cell nuclei were labeled with TO-PRO-3 (blue). Groups were analyzed by Student‘s t-test. Data are shown as mean ± SEM ± SD. * p < 0.05, *** p < 0.001. n = 5/group. Scale bar = 20 μm. Abbreviations: ONL—outer nuclear layer, OPL—outer plexiform layer, INL—inner nuclear layer, IPL—inner plexiform layer, GCL—ganglion cell layer, ILM—inner limiting membrane.