Figure 6.

Upregulation of phosphacan expression in the glaucomatous HET retina. Analyses of Acan, Bcan, and RPTPβ/ζ/phosphacan mRNA expression in the retina of WT and HET mice by RT-qPCR. We observed a significant upregulation of RPTPβ/ζ/phosphacan detected by the primer pairs RPTPβ/ζ CA (all isoforms). However, no changes in the expression of the RPTPβ/ζ receptor variants, namely RPTPβ/ζ_long and RPTPβ/ζ_short, detected by the primer RPTPβ/ζ PTP1 (receptor isoforms RPTPβ/ζ_long and RPTPβ/ζ_short) were found. Collectively, these findings indicate that phosphacan is upregulated in the HET retina. A comparable mRNA expression level was detected for Acan and Bcan. Groups were compared using a pairwise fixed reallocation and randomization test. Data are shown as median ± quartile ± minimum/maximum. * p < 0.05. n = 5/group.