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. 2021 Feb 24;10(3):169. doi: 10.3390/biology10030169

Table 1.

List of primer pairs that were used for mRNA analyses in WT and HET retinae and optic nerves via RT-qPCR. To normalize the ECM expression data, the expression of the housekeeping genes β-actin (Actb) and cyclophilin D (Ppid) was assessed. The table shows the primer names, gene names, primer sequences, GenBank accession numbers, and product sizes. Abbreviations: bp—base pairs, for—forward, rev—reverse.

Primer Name Gene Name Primer Sequence GenBank Accession Number Product Size (bp)
β-actin_for Actb ctaaggccaaccgtgaaaag NM_007393.5 104
β-actin_rev accagaggcatacagggaca
Aggrecan_for Acan ccagcctacaccccagtg NM_007424.3 66
Aggrecan_rev gagggtgggaagccatgt
Brevican_for Bcan tcaatgtctactgcttccgaga NM_007529.2 72
Brevican_rev catctgaggctgggctagag
Cyclophilin D_for Ppid aaggatggcaaggattgaaa NM_026352.4 105
Cyclophilin D_rev ctttaagcaattctgcctgga
Fibronectin_for Fn1 gccaccggagtctttactacc NM_010233.2 61
Fibronectin_rev cctcggtgttgtaaggtgga
Laminin α4_for Lama4 tgtttgttggaggtgttcca NM_010681.4 130
Laminin α4_rev ctgaccagggcagctttact
Laminin β2 _for Lamb2 tttcatttcacccacctcatt NM_008483.3 77
Laminin β2 _rev aagtctgcagaacgctccac
Laminin γ 3 _for Lamc3 gctgacctcagaagcacaca NM_011836.4 70
Laminin γ 3 _rev cacagtgctcagcccaga
RPTPβ/ζ CA_for RPTPβ/ζ gaatcctgcagagcttcctc NM_011219.2 74
RPTPβ/ζ CA_rev gtagtatccataagcccagtcca
RPTPβ/ζ PTP1_for ttgacggttccttcatgttg NM_001311064.1 101
RPTPβ/ζ PTP1_rev ttgacggttccttcatgttg
Tenascin-C_for Tnc cagggatagactgctctgagg NM_001369211.1 90
Tenascin-C_rev cattgtcccatgccagattt
Tenascin-R_for Tnr gatggaagccgcaaagag NM_022312.3 68
Tenascin-R_rev tctgacaggccctctagtcg